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In particular, substitution of platinum for carboplatin measurements in pharmacokinetic studies has occurred due to the poor sensitivity of existing HPLC assays for the parent norma ubc 97. The objectives of this study were 1 to develop and validate a sensitive and specific HPLC assay method for determination of carboplatin in plasma ultrafiltrate, and 2 to compare carboplatin AUC estimates determined using free norma ubc 97 protein-bound carboplatin versus estimates using free platinum.

Both methods yielded quantitation limits that were approximately fold more sensitive than previous HPLC-UV methods.

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Assay variability was much higher for the HPLC-PC method due to time-dependent changes in signal response caused by the degradation of the post-column reagent, sodium bisulfite. The pharmacokinetic behaviour of carboplatin during eight cycles of chemotherapy in two young patients was investigated.

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